Part:BBa_K1758120:Design

RNase E regulating protein RraA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Amplification with Gibson-primer:

• Amplification of regulator of ribonuclease activity A (RraA):

Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_fwd RraA_fwd]

Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_rev RraA_rev]

• Amplification of the pSB1C3-Backbone:

Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre]

Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf]

Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].

Source

RraA was obtained via PCR on E. coli K12 genomic DNA.